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If it is impractical to use permanently fixed markers in your volume, you can still benefit from following this procedure. However, when recalibrating, swap a scaled wand object for the custom calibration object. While your results will not be as accurate as they would be with a large calibration object, you will still benefit from the scaling involved. For details, see Step 2 of Recalibrate with scaled wand & custom calibration object.
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